In situ hybridization is a technique of detecting precise nucleotide sequences in cell preparations or tissue sections by hybridization of complementary strand of a nucleotide probe against the sequence of interest. Nucleic acids conserved in a histological specimen are detected by using a complementary probe. The usage of a fluorescent labeled probe sets apart ISH and FISH. FISH is a cytogenetic method used to detect and locate the presence or absence of specific DNA sequences on chromosomes. A DNA probe is labeled with fluorescent marker. The probe and target DNA are denatured and hybridization of the probe and target occurs. Fluorescence tag is then detected with a fluorescent microscope.

FISH is employed in various applications, from basic mapping of gene to identification of chromosomal abnormalities. Over the last few years, rise in light microscopy has increased through advancements in fluorescence techniques, thus allowing unprecedented ease, accuracy, and precision in locating, identification, and recording of data on the genetic buildup of biological samples. The influence of ISH has been largely extended by the simultaneous use of fluorescent colors. In its simplest form, FISH is primarily used to identify labeled features, as diverse fluorophores are used for hybridization. Usage of a combinations of colors helps detect varied labeled features in cells through the digital imaging microscopy. This technique of multicolor FISH, along with digital imaging microscopy, offers unmatched abilities for non-isotopic detection of many nucleic acid sequences for the examination of cell components, genes, and its chromosomes. Progress in microscope technology and probe has led to the rapid advancement of techniques for fluorescence. Application of FISH has been rising at a rapid pace in cytogenetics gene mapping, genomics, gene amplification, tumor biology, prenatal research, radiation labels, and initial biomedical research.

Spatial resolution of genomic and morphological structures can be obtained by using FISH. FISH is simple and can be implemented quickly. It also has more probe stability. The genome of a particular species, single-copy unique sequences, and entire chromosomes can be identified with the usage of varied probes. Imaging software for quantitative analysis of samples labelled with fluorescent elements was limited, as the previously existing systems for image analysis were not advanced enough to work with fluorescent samples. Reagents and probes were also not sufficient for all applications. However, new advances in dye technology of fluorescence and spin-off technology from the Human Genome Project have immense impact. Therefore, probes for all human chromosomes are available. Lack of a high level, reasonably priced FISH imaging system has adversely affected the FISH imaging system market. It has also hampered the accessibility of systems for diagnosis in healthcare settings.

FISH imaging systems are attaining popularity in diagnostics of diseases caused by bacteria or pathogens. Rise in incidence of cancers and genetic diseases, such as autism, solid tumors, lymphoma, leukemia, and other developmental syndromes, is expected to boost the FISH imaging system market. Growth in adaption of fluoroscopy, illuminators, microscopes, and CCD cameras for diagnosis of infectious diseases is providing lucrative growth opportunities for FISH imaging systems. Based on type, the FISH imaging systems market can be segmented into instruments, accessories and consumables, software and services. The instruments segment can further be bifurcated into microscopes and automated analyzers. In terms of application, the FISH imaging systems market can be segregated into cancer diagnosis and diagnosis for genetic disorder. The cancer diagnosis segment is anticipated to dominate during the forecast period due to the increase in incidence of various types of cancers.

Based on geography, the FISH imaging systems market can be split into North America, Europe, Asia Pacific, Latin America, and Middle East & Africa. North America is anticipated to dominate the global FISH imaging systems market during the forecast period, due to the availability of advanced screening processes, high health care expenditure, and rise in awareness about genetic disease in the region. A system United States providing mid-priced, commercially available FISH system for research, comprising components from a varied number of manufacturers and recent developments in microscope, image analysis, hardware, software, and accessories are driving the growth of the market in the region. Increase in focus of government organizations, such as the European Diagnostic Manufacturers Association, on development of the IVD market in Europe is fueling the FISH imaging systems market in the region. Asia Pacific is estimated to be a rapidly growing region of the FISH imaging systems market during the forecast period, led by the rise in unmet diagnostic & clinical needs in developing countries in the region.

Key players operating in the FISH imaging system market include Thermo Fisher Scientific, Inc., Leica Biosystems, PerkinElmer, Inc., Nussloch GmbH, Nikon Instruments, MetaSystems, Creative-Biolabs, Applied Spectra

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