In molecular biology, a Southern blot is a technique for finding an exact DNA sequence in a sample of DNA. This technique was first developed by E.N. Southern in 1975. It is based on the hybridization principle. Hybridization is a process by which a single stranded DNA probe converts into a double stranded DNA by combining with a single stranded target DNA. This is a specific process in which the single stranded DNA probe binds only with target DNA with complementary sequence. The probe can identify a target molecule in a mixture of millions of related but non complementary molecules. Southern blotting is defined as a technique which is used to transfer DNA fragments from an electrophoresis gel to a membrane support for immobilization of DNA fragments so the membrane brings a semi-permanent replica of the banding pattern of the gel. After the immobilization now DNA fragments, can be exposed for hybridization analysis. The number of DNA fragments required for the Southern blotting technique depends upon the size and activity of the DNA probe. Short probes tend to be more specific for target DNA. Under an ideal condition, the expected detection is 0.1 picogram of the DNA, for which the probing is done. Southern blotting is used in various molecular biology processes to identify a specific DNA in a large DNA sample; to separate a desired DNA for construction of rDNA; and to identify mutation, deletion, and gene relocation. The technique is also used in phylogenetic analysis, diagnosis of HIV-1 and infectious diseases, restriction fragment length polymorphism (RFLP), prognosis of cancer, and prenatal diagnosis of genetic diseases. This technique is also applicable in DNA fingerprinting techniques such as paternity and maternity testing, criminal identification & forensic and personal identification.
The use of Southern blotting is increasing in the field of genetics and forensic science, which is expected to boost the demand for advanced drug discovery and development. This in turn is projected to drive the growth of the Southern blotting market in the next few years. In addition, growing incidence of chronic diseases such as cancer, congenital adrenal hyperplasia, and HIV-1 and rising demand for faster diagnosis of chronic diseases are factors contributing to the market growth. Further, the use of Southern blot is rising in medical diagnosis and forensic sciences. However, factors such as unavailability of necessary devices for Southern blotting, high cost of products, and insufficient funding in developing countries are the major restraints for the growth of the Southern blotting market.
The Southern blotting market can be segmented on the basis of application, product, end-user, and region. On the basis of application, the market is categorized as medical diagnosis, genetic fingerprinting, scientific research, agricultural, and sex determination. Based on product, the market is categorized as consumables and instruments. On the basis of end-user, the market is segmented as biotechnology & pharmaceutical industries, research institutes, contract research organizations, and forensic labs.
The global market for Southern blotting is distributed over four regions: North America, Asia Pacific, Europe, and rest of the world. North America is projected to dominate the global market with the U.S. as a major contributor owing to invention of advance medications and rise in research development. Asia Pacific is projected to expand at a significant growth rate attributed to the increasing investment in advanced techniques for drug development and high patient base suffering from chronic diseases. The Europe market is growing rapidly owing to high disease occurrence and aged population.
Key players operating in the global Southern blotting market include Cleaver Scientific Ltd., Aplegan, Thermo Fisher Scientific Inc., PerkinElmer Inc., Agilent Technologies, Bio-Rad Laboratories, Pall Life Science Laboratories, and Applied Biosystems.
This study by TMR is all-encompassing framework of the dynamics of the market. It mainly comprises critical assessment of consumers' or customers' journeys, current and emerging avenues, and strategic framework to enable CXOs take effective decisions.
Our key underpinning is the 4-Quadrant Framework EIRS that offers detailed visualization of four elements:
- Customer Experience Maps
- Insights and Tools based on data-driven research
- Actionable Results to meet all the business priorities
- Strategic Frameworks to boost the growth journey
The study strives to evaluate the current and future growth prospects, untapped avenues, factors shaping their revenue potential, and demand and consumption patterns in the global market by breaking it into region-wise assessment.
The following regional segments are covered comprehensively:
- North America
- Asia Pacific
- Latin America
- The Middle East and Africa
The EIRS quadrant framework in the report sums up our wide spectrum of data-driven research and advisory for CXOs to help them make better decisions for their businesses and stay as leaders.
Below is a snapshot of these quadrants.
1. Customer Experience Map
The study offers an in-depth assessment of various customers’ journeys pertinent to the market and its segments. It offers various customer impressions about the products and service use. The analysis takes a closer look at their pain points and fears across various customer touchpoints. The consultation and business intelligence solutions will help interested stakeholders, including CXOs, define customer experience maps tailored to their needs. This will help them aim at boosting customer engagement with their brands.
2. Insights and Tools
The various insights in the study are based on elaborate cycles of primary and secondary research the analysts engage with during the course of research. The analysts and expert advisors at TMR adopt industry-wide, quantitative customer insights tools and market projection methodologies to arrive at results, which makes them reliable. The study not just offers estimations and projections, but also an uncluttered evaluation of these figures on the market dynamics. These insights merge data-driven research framework with qualitative consultations for business owners, CXOs, policy makers, and investors. The insights will also help their customers overcome their fears.
3. Actionable Results
The findings presented in this study by TMR are an indispensable guide for meeting all business priorities, including mission-critical ones. The results when implemented have shown tangible benefits to business stakeholders and industry entities to boost their performance. The results are tailored to fit the individual strategic framework. The study also illustrates some of the recent case studies on solving various problems by companies they faced in their consolidation journey.
4. Strategic Frameworks
The study equips businesses and anyone interested in the market to frame broad strategic frameworks. This has become more important than ever, given the current uncertainty due to COVID-19. The study deliberates on consultations to overcome various such past disruptions and foresees new ones to boost the preparedness. The frameworks help businesses plan their strategic alignments for recovery from such disruptive trends. Further, analysts at TMR helps you break down the complex scenario and bring resiliency in uncertain times.
The report sheds light on various aspects and answers pertinent questions on the market. Some of the important ones are:
1. What can be the best investment choices for venturing into new product and service lines?
2. What value propositions should businesses aim at while making new research and development funding?
3. Which regulations will be most helpful for stakeholders to boost their supply chain network?
4. Which regions might see the demand maturing in certain segments in near future?
5. What are the some of the best cost optimization strategies with vendors that some well-entrenched players have gained success with?
6. Which are the key perspectives that the C-suite are leveraging to move businesses to new growth trajectory?
7. Which government regulations might challenge the status of key regional markets?
8. How will the emerging political and economic scenario affect opportunities in key growth areas?
9. What are some of the value-grab opportunities in various segments?
10. What will be the barrier to entry for new players in the market?
Note: Although care has been taken to maintain the highest levels of accuracy in TMR’s reports, recent market/vendor-specific changes may take time to reflect in the analysis.