Affinity chromatography, also known as bioselective adsorption, is a type of liquid chromatography for purification of a specific molecule or a group of molecules from complex mixtures. The fundamental principle comprising the method is the isolation of biomolecules on the basis of a reversible interaction between a target molecule (protein) and a specific bioligand that has been immobilized on an inert matrix. Examples of these interactions include the binding of a fusion protein with a ligand or binding of an antibody against a specific antigen. Affinity chromatography provides high selectivity, high resolution, and high capacity for analytical analysis of proteins. Affinity chromatography has a wide range of applications in academics, pharmaceuticals, food & beverage, hospitals, cosmetics, biotechnology, nutraceuticals and others.
The global affinity chromatography reagents are significantly growing under the influence of emerging biopharmaceuticals, biotechnology and food and beverages industries. High investment in research and development by life science companies, extensive academic and industry collaborations, increased governmental funding, innovation in purification technology, advancements in the design of columns, the development of better performing reagents and rise in acquisitions/partnerships among companies are some of major divers of affinity chromatography reagents market. Furthermore, factors such as increasing demand of green chromatography, escalating usage of chromatography reagents in antibody purification, protein synthesis and drug assessment are also triggering the growth of this market. However, the presence of other advanced analytical techniques such as electrophoresis, protein crystallization, and charged ultrafiltration membranes are the major road-block for the global expansion of affinity chromatography reagents market. In addition to these, high prices of solvents such as acetonitrile, dioxane and dimethylformamide, and issues related to degradation of solid support may also hamper the growth of this market to some extent.
The global affinity chromatography reagents market has been segmented by application, technology and end user. Affinity chromatography reagents segment by applications further subcategorized into analytical chromatography reagents and preparative chromatography reagents. Analytical chromatography reagents include silylating reagent, alkylating agents, acylating reagent, ion-pairing reagents (anionic and cationic), buffers and solvents. Preparative chromatography reagents are broadly categorized into buffers, solid support, and solvents. The affinity chromatography reagents by technology is categorized into planar and column chromatography reagents based on bed shape (gas, liquid, and supercritical phase), physical state of mobile phase (adsorption, partition, ion-exchange, size exclusion and affinity), and separation mechanism. End-users of affinity chromatography reagents market are pharmaceutical companies, biotechnology companies, cosmetic industry, food and beverages industry and other end-users.
Based on geography, the affinity chromatography reagents market is segmented into North America, Europe, Asia-Pacific and Rest of the World (RoW). The North American region was recognized as the largest affinity chromatography reagents market across the globe in 2013. This market is expected to grow even further, due to robust research activities for the development of drugs and biologics and extensive financial support from governments. Europe was the second-largest market for affinity chromatography reagents owning to the opening of new production facilities and increased funding by local and national government bodies in the region. Asia-Pacific is expected to be an exponentially growing affinity chromatography reagents market during the forecast period from 2014 to 2020. This high growth is primarily due to improving economic condition in countries such as India, China and Malaysia and increase in research activities in the chromatography sector by Asian companies.
The key players in the affinity chromatography reagents include Thermo Fisher Scientific, Inc. ,G.E Healthcare ,Waters Corporation, Tosoh Corporation , EMD/Merck Millipore, Phenomenex, Inc., Sigma- Aldrich Corporation , Bio-Rad Laboratories , Pall Corporation , Life Technologies, VWR International LLC., Agilent Technologies ,W.R. Grace & Co., Becton Dickinson & Co , Regis Technologies , and Helena Laboratories.