In biotechnology, sequencing is the procedure to determine the sequence of amino acids in a protein or nucleotide sequence in the DNA. Currently, protein sequencing and gene sequencing methods are used for Microbiome sequencing. The process of identifying the order of genes in DNA is called DNA sequencing and order of amino acids in a protein is called protein sequencing. Genomic research is the most popular application of sequencing. The approaches include sequencing by synthesis (SBS), pyrosequencing, Sanger sequencing, and sequencing by ligation among others. Sequencing by synthesis or SBS is a method that utilizes DNA polymerase or ligase enzymes to extend many DNA strands in parallel. SBS methods can further be categorized as either ensemble based or single molecule–based SBS methods.
However, Sanger sequencing is a DNA sequencing technique of selective incorporation of chain terminating dideoxynucleotides by the DNA polymerase enzyme. This sequencing process was developed by Frederick Sanger and colleagues in 1977. This method was followed for more than 40 years. Ligation sequencing is a DNA sequencing method to determine the mismatch sensitivity of nucleotides in a given DNA sequence using a DNA ligase enzyme. DNA ligase enzyme helps to join ends of DNA molecules. The scientific community depends on the above approaches. Moreover, no one single platform can provide all solutions. Some platforms are more costly. The cost of maintaining and purchasing multiple sequencing platforms is high, which is a drawback of this market.
New validated target and disease diagnosis and detection are market driven for microbiome sequencing services. In-depth research work and lack of expertization are key restraints of the microbiome sequencing services market. The microbiome sequencing services market is segmented based on technology, applied research type, laboratory type, end user, and by region. Based on application, the market is further divided into sequencing by synthesis, sequencing by ligation, Sanger sequencing, pyro-sequencing, and other sequencing. In terms of research type, the microbiome sequencing market is segmented into two segments - outsourced and internal. Outsourcing is a major factor driving the microbiome sequence market. On the bases of laboratory use, the segment is further divided into wet lab and dry lab. Wet lab is a laboratory where drugs, chemicals or biological products are tested using water. Dry lab is a computer generated lab where biological or chemical data is analyzed through computer generated models. In terms of end user, the microbiome sequencing services market is segmented into research institutes, and biotechnology and pharmaceutical companies.
The microbiome sequencing services market is also segmented on the basis of geography into North America, Europe, Asia Pacific (China, India, and Japan), and Middle East & Africa. North America is considered the largest microbiome sequencing services market, followed by Europe and Asia Pacific. The U.S. was the top contributor for microbiome sequencing services market revenues. Government funding for genomic research, ongoing developments in sequencing methods, next generation sequencing (NGS) data analysis and increasing number of research studies in various fields including cancer, autoimmune disease, and rare diseases are major factors driving the microbiome sequencing services market in this region. However, the Asia Pacific region is projected to be the most promising market in the near future due to better healthcare infrastructure, economic growth, and a developing private healthcare sector with increasing awareness amongst people due to educational progress in India, China, and Japan.
Key players operating in the microbiome sequencing services market include C3J Therapeutics, Inc., uBiome, Synlogic, Blue Turtle Bio, Eligo Bioscience, Enterome, Evelo Therapeutics, Kallyope, Rebiotix, Evolve BioSystems, Taxon Biosciences, EpiBiome, MicroBiome Therapeutics, and Microbiota Therapeutics. More companies are anticipated to enter the microbiome sequencing services market in the near future.
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